منابع مشابه
Rapid Protein Separations in Microfluidic Devices
This paper describes fabrication of glass and plastic microfluidic devices for protein separations. Although the long-term goal is to develop a microfluidic device for two-dimensional gel electrophoresis, this paper focuses on the first dimension–isoelectric focusing (IEF). A laserinduced fluorescence (LIF) imaging system has been built for imaging an entire channel in an IEF device. The whole-...
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Although there have been no significant studies of the effects of different coupling chemistries, spacer arm length, or the nature of the functional group on the performance of ultrafiltration membranes, there is an extensive literature on these parameters in the field of chromatography. An optimal spacer arm ensures that the functional groups are placed a suitable distance from the surface of ...
متن کاملChromatographic Studies of Protein-Based Chiral Separations
The development of separation methods for the analysis and resolution of chiral drugs and solutes has been an area of ongoing interest in pharmaceutical research. The use of proteins as chiral binding agents in high-performance liquid chromatography (HPLC) has been an approach that has received particular attention in such work. This report provides an overview of proteins that have been used a...
متن کاملDifferent Stationary Phase Selectivities and Morphologies for Intact Protein Separations
The central dogma of biology proposed that one gene encodes for one protein. We now know that this does not reflect reality. The human body has approximately 20,000 protein-encoding genes; each of these genes can encode more than one protein. Proteins expressed from a single gene can vary in terms of their post-translational modifications, which often regulate their function within the body. Un...
متن کاملMacromolecular recognition in the Protein Data Bank
Crystal structures deposited in the Protein Data Bank illustrate the diversity of biological macromolecular recognition: transient interactions in protein-protein and protein-DNA complexes and permanent assemblies in homodimeric proteins. The geometric and physical chemical properties of the macromolecular interfaces that may govern the stability and specificity of recognition are explored in c...
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ژورنال
عنوان ژورنال: Journal of Separation Science
سال: 2016
ISSN: 1615-9306
DOI: 10.1002/jssc.201670044